| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 131 | Holdemania filiformis | CGTTTTGTTTCTGGTTTGGCG | GCCGATAGCAGTCACGATCA | 59.41 | 59.97 | 155 | 86 |
100.00%
|
50.00%
|
| Primer 132 | Holdemania filiformis | AAGACAGAAGCGCTCACGG | TCTTCGTGGCTTGCAGCA | 60.37 | 59.89 | 193 | 84 |
100.00%
|
50.00%
|
| Primer 133 | Holdemania filiformis | TGCTGGAAGGCGTGACAA | AATTCGTTGCCCTGCGGA | 59.49 | 59.97 | 154 | 84 |
100.00%
|
50.00%
|
| Primer 134 | Holdemania filiformis | GCCAACGGAGCGGAAATTC | ACGTGCTTGTGCGGATTG | 59.86 | 59.05 | 221 | 84 |
100.00%
|
50.00%
|
| Primer 135 | Holdemania filiformis | ATGTCGGCAGCGGAGTAAC | CGCGACGTCAAACTTGCA | 60.15 | 59.38 | 161 | 84 |
100.00%
|
50.00%
|
| Primer 136 | Holdemania filiformis | TGGTGATGAGCAAGCGCA | CAGAAGCTCCATGACGGCA | 59.97 | 60.08 | 199 | 84 |
100.00%
|
50.00%
|
| Primer 137 | Holdemania filiformis | AGGTTTCAGGCTTGCTGGG | ATGCGATCCGTTTGGGCT | 60.23 | 59.73 | 234 | 84 |
100.00%
|
50.00%
|
| Primer 138 | Holdemania filiformis | ACACCGCAACAGATGGCA | AACAGGCTGACCGTCATGG | 59.89 | 60.00 | 218 | 84 |
100.00%
|
50.00%
|
| Primer 139 | Holdemania filiformis | ACACCGCAACAGATGGCA | ACCGTCATGGCAACGCA | 59.89 | 59.93 | 209 | 84 |
100.00%
|
50.00%
|
| Primer 140 | Holdemania filiformis | GGACGTGGTTAGTGCGGAT | ACATTGGTTTGCGTCGCC | 59.78 | 59.36 | 247 | 84 |
100.00%
|
50.00%
|